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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata at 51 Arenas and Triangulos, on the site Antar's Notebook 3 weeks ago

Gen: COI Temp: 51°C vol: 50µl Gel: 1.6% Date: 20/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata at 51 Alacranes, on the site Antar's Notebook 3 weeks ago

Gen: COI Temp: 51°C vol: 50µl Gel: 1.6% Date: 20/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata at 51 Arcas, on the site Antar's Notebook 3 weeks ago

Gen: COI Temp: 51°C vol: 50µl Gel: 1.6% Date: 20/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata, on the site Antar's Notebook 4 weeks ago

Gen: COI Temp: 41°C vol: 50µl Gel: 1.6% Date: 14/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata, on the site Antar's Notebook 4 weeks ago

Gen: COI Temp: 41°C vol: 50µl Gel: 1.6% Date: 14/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Jay Dimond wrote a new post, Nanopore RNA protocol sheets, on the site Jay's lab notebook 1 month ago

20181113170146595 20181113170106169 20181113170024242
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47, on the site Antar's Notebook 1 month ago

Gen: COI Temp: 47°C vol: 50µl Gel: 1.6% Date: 11/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata, on the site Antar's Notebook 1 month ago

Gen: COI Temp: 40°C vol: 50µl Gel: 1.6% Date: 11/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata, on the site Antar's Notebook 1 month ago

Gen: COI Temp: 37.8 – 34°C vol: 50µl Gel: 1.6% Date: 10/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47, on the site Antar's Notebook 1 month ago

Gen: COI Temp: 47°C vol: 50µl Gel: 1.6% Date: 07/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Jörn Alexander Quent changed their profile picture 1 month ago
Jörn Alexander Quent became a registered member 1 month ago
Sam White wrote a new post, New Notebook, on the site Sam's Notebook 1 month ago
I’ve migrated my notebook to here:
- https://robertslab.github.io/sams-notebook/
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Ophiothix lineata at 38ºC, on the site Antar's Notebook 1 month ago

Gen: COI Temp: 38°C vol: 50µl Gel: 1.6% Date: 08/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4 2.5µl

Template […]
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi and L ashleyae, on the site Antar's Notebook 1 month ago

Gen: COI Temp: 47°C vol: 50µl Gel: 1.6% Date: 07/nov/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 46°C, on the site Antar's Notebook 1 month, 1 week ago

Gen: COI Temp: 46°C vol: 50µl Gel: 1.6% Date: 31/oct/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47 Alacranes, on the site Antar's Notebook 1 month, 1 week ago

Gen: COI Temp: 46°C vol: 50µl Gel: 1.6% Date: 31/oct/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47 Triangulos, on the site Antar's Notebook 1 month, 2 weeks ago

Gen: COI Temp: 47°C vol: 50µl Gel: 1.6% Date: 28/oct/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47 Arenas, on the site Antar's Notebook 1 month, 2 weeks ago

Gen: COI Temp: 47°C vol: 50µl Gel: 1.6% Date: 28/oct/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47, on the site Antar's Notebook 1 month, 2 weeks ago

Gen: COI Temp: 47°C vol: 50µl Gel: 1.6% Date: 28/oct/2018

Recipe

Each PCR tube has:

MasterMix 37.5 µl

(10µM) Ls(3)COI(FL)-F1 2.5µl

(10µM) Ls(4)COI-R2 2.5µl

Template 7.5µl
- Antar Mijail Pérez-Botello replied 1 month, 2 weeks ago
  
  The samples 132, 130 and 129 have a small band, we need to redo de PCR at lower temp 46°
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