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Brian Glanz changed their profile picture 4 hours, 2 minutes ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 47, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) of Leucothoe kensleyi at 45, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) Ophiothrix lineata at 40, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) Ophiothrix lineata at 45, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) Ophiothrix lineata at 45, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) Ophiothrix lineata at 55, on the site Antar's Notebook 1 day, 4 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification (COI) Synalphes hemphilli at 53.4, on the site Antar's Notebook 5 days, 2 hours ago
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Sam White wrote a new post, qPCRs – Ronit’s C.gigas ploidy/dessication/heat stress cDNA (1:5 dilution), on the site Sam's Notebook 5 days, 8 hours ago
IMPORTANT: The cDNA used for the qPCRs described below was a 1:5 dilution of Ronit’s cDNA made 20181017 with the following primers! Diluted cDNA was stored in his -20oC box with his original cDNA.The following […]
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Antar Mijail Pérez-Botello wrote a new post, DNA test of Ophiothrix lineata, on the site Antar's Notebook 6 days, 3 hours ago
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification Synalphes hemphilli at 54.2, on the site Antar's Notebook 6 days, 3 hours ago
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Sam White wrote a new post, Samples Received – Crassostrea virginica (Eastern oyster) tissue from Lotterhos Lab (Northeastern University), on the site Sam's Notebook 6 days, 10 hours ago
Sample sheet (Google Sheet):
Samples were stored in -80oC:
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Sam White wrote a new post, Reverse Transcription – Ronit’s C.gigas DNased ctenidia RNA, on the site Sam's Notebook 6 days, 12 hours ago
Proceeded with reverse transcription of Ronit’s DNased ctenidia RNA (from 20181016).
Reverse transcription was performed using 100ng of each sample with M-MMLV Reverse Transcriptase from Promega.
Briefly, 100ng […]
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Sam White wrote a new post, qPCR – Ronit’s DNAsed C.gigas Ploidy/Dessication RNA with elongation factor primers, on the site Sam's Notebook 6 days, 13 hours ago
After I figured out the appropriate DNA and primers to use to detect gDNA in Crassostrea gigas samples, I checked Ronit’s DNased ctenidia RNA (from 20181016) for residual gDNA.Elongation factor […]
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Antar Mijail Pérez-Botello wrote a new post, Gen amplification Synalphes hemphilli at 52.7, on the site Antar's Notebook 1 week ago
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Sam White wrote a new post, qPCR – C.gigas primer and gDNA tests with 18s and EF1 primers, on the site Sam's Notebook 1 week ago
The [qPCR I ran earlier today to check for residual gDNA in Ronit's DNased RNA] turned out terribly, due to a combination of bad primers and, possibly, bad gDNA.I tracked down some different primers for […]
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Sam White wrote a new post, qPCR – Ronit’s DNAsed C.gigas Ploidy/Dessication RNA with 18s primers, on the site Sam's Notebook 1 week ago
After DNasing Ronit’s RNA earlier today, I needed to check for any residual gDNA.Identified some old, old C.gigas 18s primers that should amplify gDNA:
- gigas18s_fw (SRID 157)
- gigas18s_rv (SRID 156)
Used some […]
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Sam White wrote a new post, DNase Treatment – Ronit’s C.gigas Ploiyd/Dessication Ctenidia RNA, on the site Sam's Notebook 1 week ago
After quantifying Ronit’s RNA earlier today, I DNased them using the Turbo DNA-free Kit (Ambion), according to the manufacturer’s standard protocol.
Used 1000ng of RNA in a 50uL reaction in a 0.5mL thin-walled […]
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Sam White wrote a new post, RNA Quantification – Ronit’s C.gigas Ploidy/Dessication RNA, on the site Sam's Notebook 1 week ago
Last Friday, Ronit quantified 1:10 dilutions of the RNA I isolated on 20181003 and the RNA he finished isolating on 20181011, but two of the samples (D11-C, T10-C) were still too concentrated.
I made 1:20 […]
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