Isolated RNA from the following samples (provided by Colleen Burge):
- Bio 26 (a LARGE amount of tissue/debris in this sample!)
- CF 2
- CF 3
- CF 17
- CF 34
- CF 35
- CF 70
- CF 71
Samples were initially flash frozen and then stored @ -80C (no preservatives used). No visible cells/tissue in all samples, except Bio 26. Samples were homogenized in 1mL TriReagent. Used the Direct-zol RNA MiniPrep Kit (ZymoResearch) according to the manufacturer’s protocol (including on-column DNase I procedure) for the remainder of the isolation. Eluted with 50uL of 0.1%DEPC-treated H2O and spec’d on NanoDrop1000.
Samples were stored in Shellfish RNA Box #5.
Samples CF 3 and CF 17 likely have insufficient total RNA for sequencing at Cornell (200ng minimum required).
UPDATE 20140514 – CF2, CF34, CF35, CF70, CF71 sent to Cornell for Illumina RNA-seq on 20140514