Category Archives: Samples Received

Samples Received

Samples Received – Acropora cervicornis (Staghorn coral) DNA from Javier Casariego (FIU)

Received 62 coral (Acropora cervicornis) DNA samples from Javier Casariego at FIU.

Spreadsheet of samples and NanoDrop concentrations provided by Javier (converted to Google Sheet): A.cervicornis_DNA_Extractions(May_2017).xlsx

Samples were temporarily stored at 4c (in FTR 213) until I can perform global methylation assessment on them tomorrow.

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Samples Received – White Abalone DNA from CDFW Shellfish Health Lab

Received white abalone (Haliotis sorenseni) DNA extracted from digestive gland, post-esophagus, and feces from Jim Moore and Blythe Marshman at the California Dept. of Fish & Wildlife.

These are intended for qPCR to assess presence of the RLOv.

Samples were stored in the big -20C in FSH 240.

 

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Samples Received – Coral DNA from Jose M. Eirin-Lopez (Florida International University)

Steven received these coral DNA samples today. Here’s his post on Google Plus (stored @ 4C in FTR 213):

 

 

Here’s the email from Jose describing the samples:

“Dear Steven, the coral DNA samples were sent today by my student Javier (cc’ed here) to your lab. Here’s an excel attached with info for the samples including concentration and treatment of the coral from which they were extracted (N, nitrogen; NP, nitrogen+phosphorous; C, control).

Please let us know when you get these in the lab so we know all is fine!

thanks!

Chema”

Here’s the spreadsheet he sent (renamed for easier identification later on – original file sent was title DNA Qbit readings), uploaded to Google Drive:

20160615_coral_DNA_Qbit_readings.xls

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Samples Received – Green Abalone Slides

Received green abalone (Haliotis fulgens) slides from Fiorenza Micheli (Hopkins Marine Station, Stanford University). These are related to the tissue she sent to us on 20160519.

Unfortunately, the writing on the casettes that were submitted to DPMG came off during processing (see note on form below). As such, the facility was unable to identify which slides came from which histology blocks. Not sure what we’ll be able to do with these. The box of slides has been stored in FSH 236 with other slide boxes.

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Samples Received – C.gigas Tissue & DNA from Katie Lotterhos

Received 6 samples from Katie today. The box was labeled and stored @ -20C.

 

Here description of the samples, via email:

Lotterhos samples (gigas) arriving tomorrow

1) Mantle tissue samples of C. gigas were collected on 20140705 (source: Pipestem Inlet) by KEL
2) Extraction on 20141028 by VG using Qiagen DNAeasy Blood and Tissue Kit
3) Beadwash on 20150720 by VG using homemade sera-mag speed beads
4) Qubit 3.0 quantification on 20151206 by KEL and the following amounts were sent:

M1: 13 uL of 386 ug/mL
M2: 13.8 uL of 326 ug/mL
M3: 13.15 uL of 380 ug/mL (solution looked cloudy)

 

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