Continuing Illumina’s generous efforts to use our geoduck samples to test out the robustness of their emerging sequencing technologies, they have requested we send them some geoduck tissue so that they can try to complete the genome sequencing efforts using the 10x genomics sequencing platform.
Tracked down and submitted four cassettes containing pinto abalone digestive gland tissue that Sean Bennett prepared, as part of his Capstone project. A full list of samples from his project is here (Google Sheet): Pinto Transcriptome
Sent them to Diagnostic Pathology Medical Group for histology processing.
She needed these samples combined in to four separate pools. However, Pool 5 was to be pooled with a total of five samples, including EPI_135 WG. She asked that the EPI_135 WG sample make up 50% of the DNA in the pool.
Using her previously determined sample concentrations, I pooled the libraries in equal quantities.
The pool volumes are high and, the calculated pool concentrations are low. Due to time limitations on our end, it was not feasible for me to SpeedVac these down to achieve the target concentration of 10nM. I’ve notified Hollie and asked her to see if Genewiz will perform that service.
Samples were shipped on dry ice to Genewiz via FedEx Standard Overnight.