Ran qPCR with black abalone samples from the 1st and 2nd experiments to see if the Xenocal prophage portal gene is detected.
Master mix calcs (Google Sheet): 20160421 – qPCR Black Abs XenoCal phage portal
All samples were run in duplicate.
Black abalone sample 08:13-2 was run as a positive control.
Plate layout, cycling params, etc. can be seen in the qPCR Report (see Results below).
Two samples failed to produce amplification: 06:6-44 and 07:12-18. All other samples amplified. Will compile this data with WSN and RLOv DNA helicase and send along to Carolyn and Stan.