Due to a wonky standard curve, I repeated the qPCR from earlier this week.
Standard curve was p18RK7 from 20161128.
All samples were run in duplicate.
Master mix calcs are here (Google Sheet): 20170619_qPCR_WSN1_Ava_Samples
Plate layouts, cycling params, etc. can be seen in the corresponding qPCR Reports (see Results below).
Baseline threshold was manually set to 580, based on Lisa’s development of the withering syndrome qPCR assay.
Well, unfortunately, it looks like the curve has gone wonky. This is two consecutive runs with the same behavior. A new curve will have to be made and tested.