The full list of samples is here (Google Sheet): 20170502_Ava_Ab_List
Standard curve was p18RK7 from 20161128.
All samples were run in duplicate.
Master mix calcs are here (Google Sheet): 20170509_qPCR_WSN1_Ava_Samples
Plate layouts, cycling params, etc. can be seen in the corresponding qPCR Reports (see Results below).
Baseline threshold was manually set to 580, based on Lisa’s development of the withering syndrome qPCR assay.
Curves look good on all runs (except the one that’s been noted and has been repeated). Will pass along to Ava and Carolyn.
This plate has a bad curve and needs to be re-run! It has been repeated below!
I’ve included this for posterity only!