Tag Archives: BX

SOLiD Sequencing Submission

Submitted the following 8 samples for SOLiD sequencing at HTGU:

 

SB unmeth C.gigas C.gigas gill pool gDNA
SB meth C.gigas C.gigas gill pool gDNA
MA Mercenaria mercenaria gill pool polyA(x2)
BX Mercenaria mercenaria gill pool polyA(x2)
Vt RE22 Vibrio tubiashii (RE22) gDNA
Vt STRAIN Vibrio tubiashii (ATCC 19106) gDNA

 

Share

Hard Clam Challenge – QPX Strain S-1 (continued from yesterday)

All clams appeared to be alive and well. Most had their siphons out when I arrived to start collecting tissues. Clams were shucked after 24hr challenge. Gill and mantle samples were collected in separate 1.5mL snap cap tubes, stored briefly on ice and transferred to -80C in “Hard Clam QPX Challenge 12/2/2009.” box.

Share

Hard Clam Challenge – QPX Strain S-1

Challenged 2 FL hard clams and 1 BX hard clam with ~100uL of unwashed, 11 day old cultures. 2 FL hard clams and 1 BX hard clam received ~100uL of QPX media, as controls. Injections were done through the hinge using 20G1 needles and aimed for the pericardial cavity. After injections, clams were left out of water for 1.5hrs, then return to small containers of sea water. They will be incubated for 24hrs.

Share

MBL Shipment – Hard Clam gill tissue in RNA Later

Received samples from Scott Lindell today. Two Ziplock bags taped together labelled “11/16/09 Clams scudders.” The bags contain 2mL screw cap tubes with small tissue samples in RNA later. One group of tubes is labelled with FL-3 # and the other group with BX-4 #. Samples will be stored at 4C to be processed later this month.

Share

MBL Shipment – Hard Clams

Received hard clams from Scott Lindell today. Two bags. One group (4 live clams, 1 empty shell) labelled as “FL” and another group (9 live clams) labelled as “BX.” Clams were transferred to separate plastic shoebox containers with sea water and sand. They were stored at 15C until ready for experiment.

Share