Ran five plates of qPCRs on bunch of DNA I extracted ealier this month.
Standard curve was p18RK7 from 20161128.
All samples were run in duplicate.
Master mix calcs are here (Google Sheet): 20171026_qPCR_WSN1_Ava_Samples
Plate layouts, cycling params, etc. can be seen in the corresponding qPCR Reports (see Results below).
Baseline threshold was manually set to 580, based on Lisa’s development of the withering syndrome qPCR assay.
Curves looked great on each plate. See images at bottom of post.
NOTE: Plate #5 had a missed rep; I think this was due to a bad pipette tip. I excluded the 3e4 point of the curve in the analysis.