Tag Archives: hatchery

Bringing Ocean Acidification System online

I was out at Manchester yesterday to help Laura out with getting things going.

tldr- Water is flowing, rising temperature seems to be an issue. This could be attributed to air temp and/or pumps.

When we arrived water temperature was up to 19C after about a week. We decided to drain down system refill, calibrate Durafets, and monitor system over next few days with respect to temperature and pH.

Here is the system draining..
[youtube https://www.youtube.com/watch?v=CtDfMPkK69E?rel=0&showinfo=0]

 

As the system drained we calibrated with NBS buffers (7-4-10). In actuality I think they were only calibrated at 7 and 4. Need to confirm calibration system with Honeywells.

Probes are designated pink, blue, green, and yellow. Two in treatment tanks and two in each of experimental systems. As we placed in 7 buffer they initially read as follows

pink – 6.6

blue – 6.98

green – 6.82

yellow – 6.89

After all were calibrated we went through buffers and just read.

img_3940

 

2016-01-29 09.51.48

 

Here are more #s

2016-01-28_09_57_31_jpg_and_2016-01-28_09_57_47_jpg_and_2016-01-28_09_57_57_jpg_1C5BDFC3.png

Tour time (if you listen closely you can hear a narration)
[youtube https://www.youtube.com/watch?v=K5xkpAAVSuI?rel=0&showinfo=0]

 

As the system started to refill with ambient water (10c) this is how the pH probes read.

2016-01-28_13_48_31_jpg_1C5BE253.png

This is without any C02 input. We then “sample calibrated” experimental system to read same pH

Camera_Uploads_1C5BE2A3.png

At the end of the day pH was set to 7.5 in treatment tank and we will monitor to see how temperature and pH holds (assuming it can adjust with high flow rates). For more on this day check out Laura’s post.

I will leave you with an inside look at treatment tanks. Note that the first tank in the video (Tank #2) has less water coming in from the head tank as compared to Tank #1.

[youtube https://www.youtube.com/watch?v=ctiNrf2DKEU?rel=0&showinfo=0]

Share

Larval Care – Pacific oyster larvae at PSRF Manchester

Continued helping on Dan’s Pacific oyster familial crosses project at the Puget Sound Restoration Fund (PSRF) hatchery at Manchester Research Station in Manchester, WA. Pacific oyster (Crassostrea gigas) families were crossed based on earlier genotyping. Here’re some pics of the day:

Share