When there was sufficient ctenidia tissue, an additional sample was stored in 75% ethanol for potential microbial analysis.
Tissue was collected from two oysters from each of the following oyster populations:
British Columbia (BC)
Oysters were sampled from each of the following tanks:
Tubes were labeled in the following fashion:
Population & Tank (e.g. OR3B)
If no tag was present on the oyster, the oyster was assigned a number (beginning at 150 and increased sequentially) and photographed with a ruler for future measurement. White colored tags were written with the number followed by the letter ‘W’ (e.g. 78W) – no tag color info was recorded for other tag colors.
Additionally, gonad developmental stage was roughly assessed: ripe, kinda ripe, or not ripe.
All info was recorded by Katherine in her notepad. All samples were retained by Katherine (not sure where she stored them).
Utensils were flame sterilized between oysters and gloves/work surfaces were washed with a 10% bleach solution between oysters.
Things look pretty good. The TruSeq DNA Methylation Library Kit (Illumina) suggests that the libraries produced should end up with concentrations >3ng/μL, but we have plenty of DNA here to make a pool for running on the HiSeq2500.
Firstly, the ladder failed to produce any peaks. Not sure why this happened. Possibly not denatured? Seems unlikely, but next time I run the Pico assay, I’ll denature the ladder aliquot I use prior to running.
Overall, the samples look as they should (see image from TruSeq DNA Methylation Kit manual below), albeit some are a bit lumpy.