Tag Archives: Martha’s Vineyard

qPCR – MV hemocyte cDNA from 20090614

Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:

3326A13

2100B07

3219A06

2100B15

3326A11

2100B12

2100A03

Plate layout/PCR set up is here. This is a second rep of these samples.

Results: Waters are clean. However, the following samples still are negative for an 18s amplicon:

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qPCR – MV hemocyte cDNA from 20090614

Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:

3326A13

2100B07

3219A06

2100B15

3326A11

2100B12

2100A03

Plate layout/PCR set up is here.

Results: Waters are clean. However, the following samples still are negative for an 18s amplicon:

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qPCRs – MV hemocyte cDNA from 20090614

qPCR – BgBL Primers

Set up qPCR with Cv_BgBL_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

 

qPCR – HMG Primers

Set up qPCR with Cv_HMG_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

 

qPCR – HSP70 Primers

Set up qPCR with Cv_HSP70_F/R primers. This is a second rep. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

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qPCRs – MV hemocyte cDNA from 20090614

qPCR – CysB Primers

Set up qPCR with Cv_CysB_F/R. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

 

qPCR -BgBp Primers

Set up qPCR with Cv_BgBp_F/R primers. Plate layout/PCR set up is here.

Results: Waters are clean. To be analyzed later with other genes that Mac has run.

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qPCR – MV hemocyte cDNA from 20090614

Set up qPCR with Cv_18s_F/R primers. Plate layout/PCR set up is here. This is a second rep. Used 1:20 cDNA plate made by Mac as template.

Results: Waters are clean. Still some samples that aren’t coming up. Will eventually run those samples using undiluted cDNA. To be analyzed later with other genes that Mac has run.

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