Tag Archives: morphometrics

Morphometrics – Olympia Oyster OA Larvae Completed

Finished measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software). That’s 2,620 larvae measured!

NOTE: Robyn previously measured 33 tubes worth of larvae, but I’m not certain where that data was saved.

All measurements by me can be found here (Google Sheet): FHL_Oly_larvae_measurements

Measured 30 larvae from each tube of the following samples:

  Box Label Color Tube Label Notes
70 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 U FB HL.L-1
71 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 V FB HL.L.2
72 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 W FB HH.L.1
73 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 X FB HH.L.2
74 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 Y SS LLH3 Many transluscent shells
75 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 Z SS LL.H.4 Many transluscent shells
76 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 AA SS LHH3 Mostly transluscent shells
77 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 BB SS LHH4
78 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 CC SS LL.L.3
79 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 DD SS LL.L.4
80 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 EE SS LH.L.3
81 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 FF SS LH.L.4
82 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LLL-1 4.3.E
83 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LLL2 4.3.F
84 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LHL-1 4.3.G
85 FHL Oly OA x DO Box 2 etOH Friedman Pink 4.4.15 FB release Low CO2 103A
86 FHL Oly OA x DO Box 2 etOH Friedman White SS Low CO2 4.4.15 release 103A All shells partially transluscent.
87 FHL Oly OA x DO Box 2 etOH Friedman Blue SS High CO2 4/5-6/15 release 103B All shells mostly transluscent.
88 FHL Oly OA x DO Box 2 etOH Friedman Pink 44/5-6/15 release FB LCO2 FB 103A

 

Example images from notes above:

 

Sample #74

 

 

Sample #75

 

 

Sample #77

 

 

Sample #85

 

 

Sample #86

 

 

Sample #87

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Morphometrics – Olympia Oyster OA Larvae Continued

Continued measurements of larvae samples from yesterday. Measured length (hinge to growing edge) and width of 30 oysters from each tube using NIS-Elements BR (10x objective and 10x nosepiece setting in software).

The measurement data can be found here (Google Sheet): FHL_Oly_larvae_measurements

Here’s the list of tubes that were measured today and their labels:

  Box Label Color Tube Label Notes
14 2015 – DO x pH Olys FHL Friedman White AM 103B-SS LH-H-2 3.31.15
15 2015 – DO x pH Olys FHL Friedman White AL 103B-SSLH-H-1 3.31.15
16 2015 – DO x pH Olys FHL Friedman White AK 3.31.15 103A-SS LL-H-2
17 2015 – DO x pH Olys FHL Friedman White AJ 3.31.15 108A-SS LL-H-1
18 2015 – DO x pH Olys FHL Friedman Blue AG 108A-SS HL-L-2 4.1.15
19 2015 – DO x pH Olys FHL Friedman White 3.30.15 SS-103A LL-H-3 Day 3 3.30.15
20 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS-103A LLH-4 3.30.15
21 2015 – DO x pH Olys FHL Friedman White 3.30.15 SS-103B Day 3 LH-H-3 3.30.15
22 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS 103B LHH-4 Day 3
23 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS-108A LL-L-3 3.30.15
24 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS 108A LLL-4 Day 3
25 2015 – DO x pH Olys FHL Friedman White 3.30.15 SS 108B Day 3 LHL-3 3.30.15
26 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS 108B LH-L-4 3.30.15 Day 3
27 2015 – DO x pH Olys FHL Friedman Blue AH 108B-SS HH-L-1 4.1.15
28 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5-103A LL-H1 SS-D5
29 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 103A LL-H-2 D5 SS
30 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 103B LH-H-1 SS D5
31 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5103B LH-H-2 D5 SS
32 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 108A LL-L-1 D5-SS
33 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 108A LL-L-2 -SS D5

 

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Morphometrics – Olympia Oyster OA Larvae

Olympia oyster larvae were measured using NIS Elements-BR (Nikon) imaging software to measure larvae length (hinge to growing edge) and width (10x objective and 10x nosepiece setting on software). Below is a screenshot of what the software, larvae, and measurement controls look like:

Measured 30 individuals from each tube.

The following samples were measured (these are the tube labels; currently not certain what shorthand labelling scheme should be used):

  Box Label Color Tube Label Notes
1 2015 – DO x pH Olys FHL Friedman White/Blue Released: 3.25.15 103B-SS Olys High CO2 3-25-15
2 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103A HLH-1 3.28.15 3/25/release
3 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103A HL-H-2 3.28.15 3.25 release
4 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103B HH-H-1 3.28.15 3/25 release
5 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103B HH-H-2 3.27.15 release
6 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 108A HL-L-1 3.27.15 3/25 release
7 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 108A HL-L2 3.27.15 3.25 release
8 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 108B HH-L-1 3.27.15 3/25 release
9 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 HH-L-2 3.27.15 3.25 release
10 2015 – DO x pH Olys FHL Friedman Blue AI 108B-SS HH-L-2 4.1.15
11 2015 – DO x pH Olys FHL Friedman White Ap 108B-SS LH-L-1 3.31.15
12 2015 – DO x pH Olys FHL Friedman White AN 108A-SS LL-L-1 3.31.15
13 2015 – DO x pH Olys FHL Friedman White AO 103A-SS LL-L-2 3.31.15

Measurement data is here (Google Sheet): FHL_Oly_larvae_measurements

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