Tag Archives: oyster

Since you’ve been gone

Soon after Ensenada I went to Chili, SICB, and PAG (in that order). The new year is often of time to let go of lingering projects, and likely I will be doing that soon. But to bring a few pending efforts to the forefront, so that I can analyze etc here is a bit of data that is (or soon will) be coming in.
Much of this is centered around the Ostrea lurida.


The first batch was 2bRAD data.
sheet

The full list of samples are here.

sc

These raw data are here.

A quick fastqc….


We also now have a fresh set of MBD-BS.. now out for sequencing.
Pregame here

pic


And just some plain old BS
pic

Details

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There is something about TEs

For purposes of proposaling and reports, I have gone back to look at a small project done in collaboration with scientist at IFREMER looking at pesticide exposure on oyster larvae methylation.


The control library had limited yield so the number of loci with data from the treated and the control library was restricted. However using a liberal 3x coverage for both, we found a total of 823 DMRs (544 hypermethylated and 279 hypomethylated).

igv


Intriguingly, when one accounts for all CGs in the genome, these DMRs are predominantly in transposable elements.

Screenshot_7_9_15__11_15_AM_1B4EF5A5.png

Reminiscent of …

biorxiv_org_content_biorxiv_early_2015_03_13_012831_full_pdf_1B4EF6FD.png


The analysis is not pretty, but here is what I have to offer.

https://github.com/sr320/nb-2015/blob/master/Cg/YE-pesticide/YE-pesticide-summary.ipynb

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First steps at an aggregated view of all DNA methylation data (updated)

Seems like I have gotten close (see here) but do not have a canonical IGV session that has all of our DNA methylation data. The goal here is to generate such a product (and publish, so I do not lose it).

All data is publicly available at

http://owl.fish.washington.edu/halfshell/index.php?dir=2015-05-comgenbro

see also data on Figshare


Updates

July 2, 2015 – added Heat Shock experiment alternative splice track
June 26, 2015 – add link to Figshare version
June 26, 2015 – updated Archive.zip
June 26, 2015 – added numerous array tracks from heat stress array experiment including 3+ tracks.
June 26, 2015 – added new track from heat stress – Heat-multi-individual-dmr.bed
June 22, 2015 – updated Archive.zip
June 22, 2015 – updated MBD-seq track gills (no bisulfite treatment) to use unique mapping (see also [this](MBD-seq track gills (no bisulfite treatment))
June 22, 2015 – Updated EE2 linkout to go to Github
June 22, 2015 – Corrected error in labelling EE2 experiment tracks
June 15, 2015 – added MBD-seq track gills (no bisulfite treatment)
June 15, 2015 – added larval pesticide treatment tracks (bisulfite treatment)
June 15, 2015 – new IGV screenshot
June 15, 2015 – added HS-Cuffdiff_geneexp.sig.gtf (differentially expressed genes from heat-shock)

 

 


Metadata

FileID Description Links
Crassostrea_gigas.GCA_000297895.1.26.gtf gtf ftp
MBD-Gill-meth MBD enriched DNA library alignment paper, info
BiGill_CpG_methylation gill methylation 5x (MBD-BS, hi output) paper
BiGill_exon_clc_rpkm Corresponding exon-specific gene expression paper
BiGo_CpG_methylation male gamete methylation 5x (hi output) paper
M1 male gamete methylation 5x preprint
M3 male gamete methylation 5x preprint
T1D3 72hpf larvae from M1 methylation 5x preprint
T1D5 120hpf larvae from M1 methylation 5x preprint
T3D3 72hpf larvae from M3 methylation 5x preprint
T3D5 120hpf larvae from M3 methylation 5x preprint
Heat-multi-individual-dmr.bed Heat Stress (13 locations) common signal notebook
2M_3plusmerge_Hyper.bed merging adj probes to single interval notebook
2M_3plusmerge_Hypo.bed merging adj probes to single interval notebook
4M_3plusmerge_Hyper.bed merging adj probes to single interval notebook
4M_3plusmerge_Hypo.bed merging adj probes to single interval notebook
6M_3plusmerge_Hyper.bed merging adj probes to single interval notebook
6M_3plusmerge_Hypo.bed merging adj probes to single interval notebook
2M_Hyper_3plusAdjactentProbes.gff 3+ adjacent probes notebook
2M_Hypo_3plusAdjactentProbes.gff 3+ adjacent probes notebook
4M_Hyper_3plusAdjactentProbes.gff 3+ adjacent probes notebook
4M_Hypo_3plusAdjactentProbes.gff 3+ adjacent probes notebook
6M_Hyper_3plusAdjactentProbes.gff 3+ adjacent probes notebook
6M_Hypo_3plusAdjactentProbes.gff 3+ adjacent probes notebook
2M_sig Heat stress DMRs (array), ind.#2 notebook, draft
4M_sig Heat stress DMRs (array), ind.#4 notebook, draft
6M_sig Heat stress DMRs (array), ind.#6 notebook, draft
HS-Cuffdiff_geneexp.sig.gtf Heat stress differentially expressed genes notebook
HS-Cuffdiff_altsplice.bed Heat stress alternatively spliced genes notebook
2M.bedgraph.tdf RNA-seq from ind.#2 above – pretreament notebook, draft
4M.bedgraph.tdf RNA-seq from ind.#4 above – pretreament notebook, draft
6M.bedgraph.tdf RNA-seq from ind.#6 above – pretreament notebook, draft
2M-HS.bedgraph.tdf RNA-seq from ind.#2 above – post-heatshock notebook, draft
4M-HS.bedgraph.tdf RNA-seq from ind.#4 above – post-heatshock notebook, draft
6M-HS.bedgraph.tdf RNA-seq from ind.#6 above – post-heatshock notebook, draft
mgaveryDMRs_112212.gff EE2 exposure DMRs (array) paper
A01.smoothed EE2 exposure array data – input versus input paper
A02.smoothed EE2 exposure array data – EE2 vs control paper
A03.smoothed EE2 exposure array data – EE2 vs control (dyeswap) paper
YE_mixHYPER.bed DMRs in pesticide exposed larvae (hypermethylated)
YE_mixHYPO.bed DMRs in pesticide exposed larvae (hypomethylated)
YE_mix_22smCG3x larvae (mix pesticide exposed) methylation
YE_control_22smCG3x larvae (control) methylation

screenshot

anyone should be able to render this in IGV with this session file:

http://owl.fish.washington.edu/halfshell/2015-05-comgenbro/igv_session.xml


This work was supported in part by the National Science Foundation (NSF) under Grant Number 1158119 awarded to SR Roberts

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