Tag Archives: PSN 0M #2

qPCR – RLOv DNA Helicase 2011 Water Filter DNA

Since we have a working qPCR for detecting the withering syndrome bacteriophage (RLOv), Carolyn wanted to see how detection/quantification compared to withering syndrome detection/quantification on water samples collected from various farms and their nearest wild abalone site.

DNA samples used were extractions from water filters collected for the Ab Endo Project in 2011.

Ran qPCR using the RLOv DNA helicase standard curve from 20151106.

All samples were run in duplicate.

Master mix calcs are here (Google Sheet): 20151228 – qPCR RLOv 2011 H2O Filters

Plate layout, cycling params, etc. can be found in the qPCR Report (see Results below).

qPCR LABEL FULL DESCRIPTION FARM/WILD SITE NEAREST FARM/WILD SITE
CI_SCI_PC_1A Painted Cave SCI 1A Wild The Cultured Abalone
CI_SRI_PC_1B Painted Cave SCI 1B Wild The Cultured Abalone
CI_SCI_PC_2B Painted Cave SCI 2B Wild The Cultured Abalone
CI_SCI_PC_2A Painted Cave SCI 2A Wild The Cultured Abalone
CI_SCI_PRIS_1A Prisoner’s 1A Wild The Cultured Abalone
CI_SCI_PRIS_2A Prisoner’s 2A Wild The Cultured Abalone
CI_SCI_PRIS_1B Prisoner’s 1B Wild The Cultured Abalone
CI_SCI_PRIS_2B Prisoner’s 2B Wild The Cultured Abalone
RM_0M_SW Rancho Marina 0M SW Wild The Abalone Farm
RM_0M_SW_#1 Rancho Marina 0M SW #1 Wild The Abalone Farm
PSN_0M_#2 Pt. Sierra Nevada 0M #2 Wild The Abalone Farm
PSN_0M_#1 Pt. Sierra Nevada 0M #1 Wild The Abalone Farm
CARMEL_0M_2 Carmel 0M 2 Wild American Abalone
AMER_DRAIN_1A American Abalone Drain 1A Farm Carmel
AMER_DRAIN_2A American Abalone Drain 2A Farm Carmel
AMER_DRAIN_1B American Abalone Drain 1B Farm Carmel
AMER_DRAIN_2B American Abalone Drain 2B Farm Carmel
AMER_0M_OUT_1A American Abalone 0M Outfall 1A Farm Carmel
AMER_0M_OUT_2A American Abalone 0M Outfall 2A Farm Carmel
AMER_0M_OUT_1B American Abalone 0M Outfall 1B Farm Carmel
AMER_0M_OUT_2B American Abalone 0M Outfall 2B Farm Carmel
TAF_DRAIN_DUP2B The Abalone Farm Drain Dup 2B Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_2C The Abalone Farm Drain Dup 2C Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_DUP2D The Abalone Farm Drain Dup 2D Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_1D The Abalone Farm Drain Dup 1D Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_DUP2A The Abalone Farm Drain Dup 2A Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_DUP1A The Abalone Farm Drain Dup 1A Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_1B The Abalone Farm Drain Dup 1B Farm Pt. Sierra Nevada/Rancho Marina
TAF_DRAIN_1C The Abalone Farm Drain Dup 1C Farm Pt. Sierra Nevada/Rancho Marina
CAB_N.OUT_1A The Cultured Abalone North Outfall 1A Farm Santa Cruz Islands
TCA_N.OUT_1B The Cultured Abalone North Outfall 1B Farm Santa Cruz Islands
CAB_N.OUT_1C The Cultured Abalone North Outfall 1C Farm Santa Cruz Islands
CAB_N.OUT_1D The Cultured Abalone North Outfall 1D Farm Santa Cruz Islands
TCA_S.OUT_1A The Cultured Abalone South Outfall 1A Farm Santa Cruz Islands
TCA_S.OUT_1B The Cultured Abalone South Outfall 1B Farm Santa Cruz Islands
CAB_S.OUT_1C The Cultured Abalone South Outfall 1C Farm Santa Cruz Islands
CAB_S.OUT_1D The Cultured Abalone South Outfall 1D Farm Santa Cruz Islands

Results:
qPCR Report (PDF): Sam_2015-12-28 15-44-35_CC009827.pdf
qPCR Data File (CFX96): Sam_2015-12-28 15-44-35_CC009827.pcrd

Overall, data looks good. Will enter copy numbers into the Ab Endo master sheet for later analysis (Google Sheet): Ab Endo Samples

 

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