Tag Archives: RNA Pico

Bioanalyzer Data – Geoduck RNA from Histology Blocks

I received the Bioanalyzer data back for the geoduck foot RNA samples I submitted 20150422. The two samples were run on the RNA Pico chip assay.

 

Results:

Bioanalzyer 2100 Data File (XAD): SamWhite_Eukaryote Total RNA Pico_2015-04-23_13-04-16.xad

Data file requires 2100_Expert_B0208_SI648_SR2 version of the software (Windows).

Gel Representation

 

Electropherogram

 

The samples look really good! As we’ve seen previously in shellfish RNA, there is a single, prominent rRNA band/peak with very little degradation (smearing and/or no prominent peak/band).

Will proceed with RNA isolation from the remaining histology blocks.

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Bioanalyzer Total, mRNA and post-fragmentation SOLiD Libraries – Abalone pools

0.5uL of fragmented mRNA from each library (combined with 0.5uL) was run on Agilent Bioanalyzer 2100 using RNA Pico chips/reagents according to Agilent’s protocol.

Results:

Total RNA shows a single, distinct rRNA band, along with some low-molecular weight RNA (i.e. degraded) in both total RNA samples. mRNA samples exhibit the expected “smear” that spans a large range of molecular weights. Both mRNA samples also show residual rRNA bands, but their concentrations should be extremely low. Fragmented samples show the expected strong band of low-molecular weight RNA. The CE frag sample exhibits some larger banding, which is probably background signal (compare to the empty lane labelled “Sample 7″).

Will proceed with rest of library procedure for both fragmented samples.

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