We need to send half of each sample that we have from Sean Bennett’s Capstone project to Alyssa Braciszewski at UC-Irvine.
This is quite the project! There are ~75 samples, and about half of those are tissues (presumably digestive gland) stored in RNAzol RT. The remainder are RNA that has already been isolated. Additionally, tube labels are not always clear and there are duplicates. All of these factors led to this taking an entire day in order to decipher and process all the samples.
I selected samples from only those that I was confident in their identity.
I aliquoted 25μL of each RNA for shipment to Alyssa.
Tissue samples were thawed and tissue was cut in half using razor blades.
Planning to send samples on Monday.
Lisa has already assembled a master spreadsheet to try to keep track of all the samples and what they are (Google Sheet): Pinto Transcriptome
Today we sampled the geoduck (Panopea generosa) for genome sequencing. Here is how things went down.
It was an early morning for the clam, peaking out to see a glorious sunrise on the porch.
From there it was off to the lab.
After cleaning the surfaces, Brent sampled tissue.
We started out started out targetting the foot and adductor muscles. These tissues were steriley removed and then rinsed in 1% bleach, followed by Nanopure water. This tissue will be used for genome sequencing as we predict least amount of associated taxa.
Remaining tissues were taken, primarily for RNA-seq and divided into two boxes.
Tubes were labeled on cap with tissue type.
Here is what Box 1 looks like.
Box 2 looks the same however it does not have a heart or style sample.
The only surpise was in sampling, labial palps were identified after we had already sampled a pair.