Ran WSN1 and RLOv DNA helicase qPCRs on the black abalone DNA I extracted yesterday to assess whether or not these samples are RLO+/- and RLOv+/-. According to Carolyn (and this spreadsheet), they should all be RLO+/RLOv-, which is what I need in order to proceed with testing samples with the XenoCal prophage portal primers.
WSN1 Master Mix Calcs (Google Sheet): 20150330 – qPCR Black Ab 08:13 WSN1 Check
RLOv DNA Helicase Master Mix Calcs (Google Sheet): 20160330 – qPCR Black Ab 08:13 RLOv check
All samples were run in duplicate.
Plate layout, cycling params, etc. are in the qPCR Report (see Results section below).
RLOv DNA helicase standard curve from 20151224.
WSN p18RK7 standard curve from 20160316.
Baseline thresholds were set to the following values for each assay (RLOv threshold determined by me on 20160128; WSN1 threshold determined by Lisa):
RLOv DNA helicase: 580.5
All samples are RLO+/RLOv-. This is great and can proceed with checking them with the XenoCal prophage portal primers.
RLOv DNA Helicase Standard Curve
RLOv DNA Helicase Amplification (Green = Std Cuve, Blue = Samples)
WSN1 Standard Curve
WSN1 Amplification (Blue = Standard Curve, Black = Samples)