Our understanding of immunity in invertebrates is limited by the tools developed to study it at this time. Today we had lectures from Carolyn and Steven on the host response to stress and infection, showing how little we know about possible adaptive immune responses in invertebrates. These two lecture highlighted how far we still have the go in understanding the innate immune and adaptive immune responses in invertebrates.
In the lab we focused on PCR on the laby primers Lisa brought to test and the sea star primers from the Hewson lab for the potental SSWD pathogen. Both will be used for qPCR, but we need to know first that they amplify so ran traditional PCR today.
Collin and I tested the sea star wasting disease primers. Samples were from the 2 non-symptomatic and 2 symptomatic pisaster collected from the field site we surveyed. Tissue was removed from the tube feet, dermal tissue, and digestive tract. Recipe used for PCR is noted in the methods handout for laby. It was provided by Lisa.
The gel show bands in the negative control for all three sets of SSWD samples. The samples will need to be rerun. Our groups samples included rows 1-13 in the lower half of the gel. There is an uper band which may be product. Tomorrow we will examine on more detail.
Oyster/SSWD pilot study:
10 oysters outplanted on the FHL dock were removed at 24 hours exposure. 10 oysters from each of the OA lab replecates were removed at 48 hours exposure. Oysters were rinsed on DO water on removal and frozen whole in the -80. The experiment will be taken down tomorrow after 72 hours exposure.