And so it begins…

Today started with a great lecture about fungal, bacterial and polychaete infections in invertebrates. Particularly in abalone and oysters. I especially enjoyed learning about the history of how all the populations of these different microorganisms have been affected. A common theme seems to be that things get worse for microorganisms when the temperature rises. After the lecture we spent the rest of the day setting up our experiment and finishing our PCR from yesterday. Unfortunately Rachel and my ITS PCR failed yet again. Now at the turn of the tide. Sufficiently humbled by 2 erroneous PCRs. I fall upon my micropipett. The good news is that Sarah and Monica’s ITS cultured laby PCR product did get a visible band so there is that. It might be worth trying just one more time and being meticulously careful and superstitious and of course ever so humble. Towards the end of the day we all examined healthy and unhealthy seastar slides under the microscope and Alison showed me the digestive tract, a bunch of seastar sperm, and tube feet muscle. However, we were unable to see anything strikingly out of place in the sick seastars compared to the healthy ones. I am excited to learn more about the wasting disease.

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