Sea Star Immune Response

Some pretty pictures from today. These are showing the genes differentially expressed in the exposed vs. the non-exposed sea stars. The immune response is lighting up clearly. After making this I worked on coding to get through the enrichment but had to stop when SQLShare stopped working, likely due to all the pressure our class was putting on it running these huge files!

I worked on finding the literature on sea star immunity and the genes involved in regulating echinoderm structural integrity, as well as some of the genes identified top hits today.

We heard great talks from Reyn, Ruth, Rachel and Cody tonight. Really interesting stuff!

I have a theory that the pathogen is disrupting the mutable collagenous tissues (MCT) somehow. Interested in getting the groups thoughts on this. In Lauren’s top ten down regulated and up regulated genes there are several involved in MCT and extracellular matrix: Heparanase, Collagenase 3 (MMP-13!), Fibropellin-3, and Myocilin. MCT is involved especially exciting to see on here as it is involved in the tensile function of the stars. limb loss, and regeneration. Below is the PhD and published paper of a researcher who did a lot of work on MCT in echinoderms.

 

Ribeiro_etal_2012PLOS_Ecinoderms_MutableCollagenousTissue

Ribeiro_PhDThesis_UniversidadedoPorto2011_EcinodermMutableCollagenousTissues

Hillier_etal_Genomics2007_OlfactomedinProteinsUrchins

Now, I know these two are about vertebrates but bear with me. Take a look at the cellular mechanisms involved and the pathogen connection. It is pretty cool.

Cardiovasc Res-2006-Rutschow-646-56-1

Cardiovasc Res-2002-Li-235-47

Another interesting gene is Cytochrome P450 monooxygenase. Sure there is something newer on this but still a good paper. This one has xenobiotic functions and is lower in the exposed animals.

Cytochrome P450 monooxygenase system in echinoderms

Here are a few more interesting papers on echinoderm ceolomocytes.

Sharlaimove_etal.CellTissueRes_2014_StarfishCoelomicCells

Franco_etal_Proteomics2011_ProteomeSeaStarCoelomocytes

Revigo figures from today! These are from the differentially expresed genes in the sea star Look at the bright red dot showing up for immune response.

Revigo figures from today! These are from the differentially expressed genes in the sea star Look at the bright red dot showing up for immune response.

Screen Shot 2014-08-07 at 10.31.54 PM

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