PCR-ISH

In an effort to run in situ hybridizations (ISH) on both coral and sea star samples, we tested 9 sets of primers today and multiple temperatures.

I ran Eubacterial primers, 27F & 1522R, and Ehrlichiae primers on coral samples. The coral DNA is from Sarah’s A. cervicornis white band samples. Below are the master mixes and annealing temps that were tested for the Ehrlichiae outside of the 55C temperature that we ran the Eubacteria primers on.

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