Today started with a beautiful morning drive to false bay. The tide was lower today and the wind less intrusive so we were able to compleat all the work planned. Fieldwork was led by Maya Groner and Drew Harvell.
1. Prevelence of disease in mid level plot (n=10 2nd longest blades removed each 10 meter from 0-50). The blades were measured and level of disease assessed back in the lab.
2. Collected Zostera marina shoots for Maya’s experiment.
3. Assessed mid level plot density (# plants on frame at 0, 25, and 50 meters).
3. Located the culling experiment plots and removed blades from center of each. There was noticeably more smithora present at this plot compared to the one we visited yesterday on Shaw. At this site there were 2 sets of plots.
Plot 1: Control1-cull1-cull2-control2, Plot 2: control1-cull1-cull2-control2
Today I also started work on a pilot study to determine if bivales filter the sea star wasting disease pathogen out of the water.
1. Juvanile oysters (1-3 cm) were out planted at Eastsound Dock (high SSWD) and Cresent Bay (low SSWD). N=10 were left for duration of the low tide and 10 left for 24 hours. Oysters were placed on mesh bags just below the surface of the water, next to where pisaster are found with disease. Many thanks the Amy Henry and Natalie Rivlin for thier help placing the bags at Orcas Island. They returned at 6 pm with the first set of oysters, which I then removed from shell, measured, weighed wet tissue, and froze at -80.
I set the experiment up in the ocean acidification lab. It will only run a few days but if the data turns out interesting may be the basis of a larger study.
I will include more on the experimental design tomorrow. Basic set up: 3 control (no stars) and 3 experiment tanks (symptomatic stars), 40 oysters in each bag, 10 oysters removed at each of 4 time points after which experiment is ended. The field and lab data will then be looked at with disease prevelence at the field sites and data from clams.
Tomorrow I will be heading over to Orcas Island to retrieve the remaining oysters (24 hour exposire) and out planting a bag at the FHL dock.