Gen amplification (COI) Ophiothrix lineata at 45

Gel_20_10_2018-3_GenCOI_Olineata_at_45-01Protocolo_termociclador_Lken1-01

Gen: COI       Temp: 45ºC          Vol: 50µL        Gel: 1.6%    Date: 21-Oct-2018    Specie: O. lineata

Recipe

Each PCR tube has:

MasterMix 37.5µl

(10µM) Olin F1 2.5 µl

(10µM) Olin R4 2.5µl

Template 7.5µl