Gen amplification (COI) of Ophiothix lineata at 38ºC

Gel_08_11_2018-1_GenCOI_Olin_at_38-01 Protocolo_termociclador_38-01

Gen: COI           Temp: 38°C      vol: 50µl         Gel: 1.6%     Date: 08/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for each sample

Code-1 = 1µl template + 99µl Nuclease Free H20

Code-10 = 10µl temlate + 90µl Nuclease Free H2O

Code-20 = 20µl template + 80µl Nuclease Free H2O

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