Gen amplification (COI) of Ophiothix lineata

Gel_09_11_2018-1_GenCOI_Olin_Grad_37_8_to_34-01Protocolo_termociclador_Rampa-01

Gen: COI           Temp: 37.8 – 34°C      vol: 50µl         Gel: 1.6%     Date: 10/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Code-20 = 20µl template + 80µl Nuclease Free H2O