Category Archives: Geles

Gen amplification (COI) of Ophiothix lineata at 51 Arenas and Triangulos

Gel_20_11_2018-3_GenCOI_Olin_at_51 Protocolo_termociclador_51-01

Gen: COI           Temp: 51°C      vol: 50µl         Gel: 1.6%     Date: 20/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Sample  = 20µl template + 80µl Nuclease Free H2O

Gen amplification (COI) of Ophiothix lineata

Gel_15_11_2018-1_GenCOI_Olin_at_42

Protocolo_termociclador_42-01

Gen: COI           Temp: 41°C      vol: 50µl         Gel: 1.6%     Date: 14/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Code-20 = 20µl template + 80µl Nuclease Free H2O

 

Gen amplification (COI) of Ophiothix lineata

Gel_14_11_2018-4_GenCOI_Olineata_at_42-01Protocolo_termociclador_41-01

Gen: COI           Temp: 41°C      vol: 50µl         Gel: 1.6%     Date: 14/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Code-20 = 20µl template + 80µl Nuclease Free H2O

Gen amplification (COI) of Ophiothix lineata

Gel_11_11_2018-2_GenCOI_Olineata_at_40-01 Protocolo_termociclador_Olin_at_40-01

Gen: COI           Temp: 40°C      vol: 50µl         Gel: 1.6%     Date: 11/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Code-20 = 20µl template + 80µl Nuclease Free H2O

Gen amplification (COI) of Ophiothix lineata

Gel_09_11_2018-1_GenCOI_Olin_Grad_37_8_to_34-01Protocolo_termociclador_Rampa-01

Gen: COI           Temp: 37.8 – 34°C      vol: 50µl         Gel: 1.6%     Date: 10/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Code-20 = 20µl template + 80µl Nuclease Free H2O

Gen amplification (COI) of Ophiothix lineata at 38ºC

Gel_08_11_2018-1_GenCOI_Olin_at_38-01 Protocolo_termociclador_38-01

Gen: COI           Temp: 38°C      vol: 50µl         Gel: 1.6%     Date: 08/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for each sample

Code-1 = 1µl template + 99µl Nuclease Free H20

Code-10 = 10µl temlate + 90µl Nuclease Free H2O

Code-20 = 20µl template + 80µl Nuclease Free H2O