Category Archives: Good

Gen amplification (COI) of Ophiothix lineata at 51 Arenas and Triangulos

Gel_20_11_2018-3_GenCOI_Olin_at_51 Protocolo_termociclador_51-01

Gen: COI           Temp: 51°C      vol: 50µl         Gel: 1.6%     Date: 20/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Sample  = 20µl template + 80µl Nuclease Free H2O

Gen amplification (COI) of Ophiothix lineata

Gel_15_11_2018-1_GenCOI_Olin_at_42

Protocolo_termociclador_42-01

Gen: COI           Temp: 41°C      vol: 50µl         Gel: 1.6%     Date: 14/nov/2018

Recipe

Each PCR tube has:

MasterMix  37.5 µl

(10µM) Olin-F1 2.5µl

(10µM) Olin-R4  2.5µl

Template 7.5µl

IMPORTANT NOTE:

In this teast we perfomed some DNA dilutions for the template

Code-20 = 20µl template + 80µl Nuclease Free H2O