Posted by & filed under Ostrea lurida.

The first batch of sequencing came into today to verify sequence of Olympia oyster qPCR primers.

1) imported .ab1 files into CLC,

2) trimmed “CARM” sequences

Remove old trimming = Yes
Quality trimming = Yes
Quality limit = 0.05
Ambiguity trimming = Yes
Ambiguity limit = 2
Vector trimming = No
User vector trimming = No

3) aligned to comp7220_c0_seq2


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