Integrating last bit of qPCR data into master datasheet. This includes 5 runs post 8/15.
likely error above – second EF1 was 18s test.
Looks nice, corrected…
Correction is fine, but mechanical reps have some issues
As noticed in last batch of analysis these need to be checked at raw data level
Thoughts on normalizing gene…
Noting that one EF1 rep was thrown out for mechanical (see above). Here is a crude look at EF1, actin, and 28s respectively…