Quick Carmalign

Posted by & filed under Ostrea lurida.

The first batch of sequencing came into today to verify sequence of Olympia oyster qPCR primers. 1) imported .ab1 files into CLC, 2) trimmed “CARM” sequences 3) aligned to comp7220_c0_seq2

A short sequence on short sequence

Posted by & filed under Tutorial.

A quick tutorial to check out primers on NCBI to see what the product size should be and how specific they are. Old school way is just take the primer with NNNs in the middle and blast. TATTCGTTGGAGACTTTATAACCCTNNNNNNNNNNNNNNNNNNNNNAAGGCTTAGAATTGCAAGGTCTATA You will get an output as such…. Scroll down to the alignments…. And look at the coordinates……. Read more »