Today I determined DNA yield for several samples that I had not yet tested with the Qubit. Here are the results (along with previous Nanodrop results), which can also be found in this spreadsheet:
|Sample #||Nanodrop DNA conc. (ng/ul)||Qubit conc. (ng/ul)||Sample volume||Total DNA yield (ng)|
I also evaluated DNA quality on a gel for several samples. Here is the gel:
As can be seen here, some samples look pretty good, some look ok, and some look like crap. Why such a range? I have no idea, they were all preserved and extracted the same way. Past95 and past19 in particular are particularly bad. These samples should be re-extracted.