November will be an important month. Here are my goals:
1. Prep for Belize trip
- Prepare DMSO and RNA later in 50 ml tubes
- Order supplies: decitabine, magnetic stirrers, heaters, etc
- Anything else??? Bring waterpik, hemacytometer?
2. Belize trip goals
- Collect transplanted P. astreoides, save separate samples for DNA and morphology
- Run short term (3-7 days) gene expression experiment in backreef
- Run two week heat stress experiment in aquaria
- Possibly collect more P. porites samples
- Consider other side projects
3. Continue analysis of RADseq data
- Things to consider: allow missing data? test for outlier loci? how to deal with EpiRAD data?
- Goal to have mostly complete analysis by the end of the month (also end of class)