mRNA Isolation – Gigas BB and DH samples previously treated with Ribominus Kit (by Mac)

Was given 1ug of each of these two RNA samples and processed them with Promega’s PolyA Tract Kit according to protocol. After elution, the samples were EtOH precipitated @ -20C for 30mins, pelleted 30mins 16,000g for 30mins, 4C. Supe removed, RNA washed with 1mL 70% EtOH and spun 15mins 16,000g, 4C. Supe removed. Resusupended in 15uL of 0.1% DEPC-H2O and spec’d.

Results: No measurable amount of RNA in either sample. Samples were stored @ -80C.

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