EtOH Precipitation – Rick’s trout Ribosomoal-depleted RNA for SOLiD WTK (from today)

The “control” and “poly I:C” samples prepared earlier today were EtOH precipitated in preparation for fragmentation.

Added the following to each sample:

  • 18uL 5M ammonium acetate
  • 1uL glycogen
  • 2 vols. of 100% EtOH (74uL)

Samples were incubated O/N @ -80C.

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