Lexie’s PCR with this primer set and a pool of Mercenria cDNA has yielded contamination in all of her waters. Performed two sets of PCR: one with her existing primer working stocks and the other with a fresh aliquot of primer working stocks. Used my own reagents/water. PCR set up and cycling params are here. PCR ran O/N.
Lane 1 – 100bp ladder
Lane 2 – H2O (Lexie’s primer stocks)
Lane 3 – H2O (Lexie’s primer stocks)
Lane 4 – cDNA (Lexie’s primer stocks)
Lane 5 – H2O (fresh primer stocks)
Lane 6 – H2O (fresh primer stocks)
Lane 7 – cDNA (fresh primer stocks)
All water samples look clean and there’s a nice bright band in the cDNA samples. Lexie’s contamination issue is probably a technique issue and not one of reagent contamination.