Colony PCR – Colonies from COX1 Genomic Cloning (from 20110411)

Ran colony PCR on various colonies produced from cloning on 20110411. All colonies were picked, re-streaked on Kan50 plate(s) and PCR’d. Master mix calcs are here. Cycling params:

  • 95C – 10mins

40cycles of:

  • 95C – 30s
  • 55C – 30s
  • 72C – 3mins
  • 72C – 10mins


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