Reverse Transcription – Dave’s Manila Clam (Venerupis philippinarum) DNased RNA from 20120307 and 20120302

Performed reverse transcription on 1.5ug of DNased RNA in a 75uL reaction, using oligo dT primers. All reagents were scaled appropriately (based on Promega’s M-MLV RT protocol). Samples were prepared in a plate and stored @ -20C. Plate layout and all reverse transcription calcs are here:

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