Combined the following libraries in equal quantities (17ng each) to create a single, multiplexed sample for sequencing (LSU_Oil_01):
- HB2 – 1 (ATCACG)
- HB16 – 3 (TTAGGC)
- HB30 – 4 (TGACCA)
- NB3 – 5 (ACAGTG)
- NB6 – 6 (GCCAAT)
- NB11 – 7 (CAGATC)
Quantified pooled libraries using the Quant-iT dsDNA BR Kit (Invitrogen) with a FLx800 plate reader (BioTek). Used 1μL of the pooled sample, run in duplicate. Used 1uL of standards, run in duplicate.
pooled libraries = 6.575ng/μL
Will submit to University of Oregon Genomics Core Facility for 100bp, single end Illumina HiSeq2500 sequencing. They need 10nM of sample. For a library with average size range of 300-400bp, this requires a sample volume of 20uL with a concentration of 2.28ng/μL in a solution of 0.1% Tween20 in Buffer EB (Qiagen).
Combined 6.94μL of pooled libraries with 13.06 of 0.1% Tween20/EB solution.
Submitted sample LSU_Oil_01 to University of Oregon Genomics Core Facility via O/N FedEx on dry ice. Sample was assigned order # 2112.