The DNA extracted and sheared on 20150109 was in a volume too great to proceed with bisulfite conversion. Dried the samples to complete dryness in a SpeedVac (~4hrs). Reconsitituted DNA in 24μL of NanoPure water. Will bisulfite convert tomorrow.
|SAMPLE ID||DATE||TREATMENT (ppm)||# LARVAE||TOTAL DNA (ng)|
Updated the quantification spreadsheet with a column labeled “[New](ug/uL)” that calculates the new concentrations of the above samples in the 24μL volume.