Bisuflite NGS Library Prep – C.gigas larvae OA bisulfite DNA (continued from yesterday)

Continued Illumina library prep of bisulfite-treated DNA samples (400ppm and 1000ppm; from 20150114)  with Methylamp DNA Modification Kit (Epigentek). Performed bead clean up immediately after End Repair.

PCR cycles: 14

No other changes were made to the manufacturer’s protocol.

Epigentek Barcode Indices assigned, per their recommendations for using two libraries for multiplexing:

400ppm – barcode #6 – GCCAAT

1000ppm – barcode #12 – CTTGTA

The two libraries were stored @ -20C and will be quantified tomorrow.


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