Samples Received – C.virginica gonad tissue from Katie Lotterhos

Received and stored @-80C in rack 8, row 5, column 5.

The following information was sent with the samples:

Sample.ID Date Temp pCO2 Notes
031 26-Aug-2016 15 400
032 26-Aug-2016 15 400
033 26-Aug-2016 15 400
034 26-Aug-2016 15 400
035 26-Aug-2016 15 400 All sample sent; it will be in 2mL screw-cap vial
036 26-Aug-2016 15 400
103 26-Aug-2016 15 2800
104 26-Aug-2016 15 2800
105 26-Aug-2016 15 2800
106 26-Aug-2016 15 2800 All sample sent; it will be in 2mL screw-cap vial
108 26-Aug-2016 15 2800

Katie sent this additional info in an email to Steven and me:

These C. virginica samples were exposed to control (400, 6 samples) and OA (2800, 5 samples) conditions for ~4 weeks at 15C. Gonad was carefully extracted by peeling back the outer membrane, flash frozen in liquid N, and placed in -80C (until today when we removed it). During sampling, it was difficult to get a lot of what we considered “pure” gonadal tissue. We sent you ~1/2 of the amount of tissue we have for all samples except for the two samples which were very low and we sent you all the tissue sample we have. Each should be about 10-20 mg of tissue, which I’m worried is not enough for MBD-BS seq. Fingers crossed.

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