Category Archives: Samples Received

Samples Received – C.virginica gonad tissue from Katie Lotterhos

Received and stored @-80C in rack 8, row 5, column 5.

The following information was sent with the samples:

Sample.ID Date Temp pCO2 Notes
031 26-Aug-2016 15 400
032 26-Aug-2016 15 400
033 26-Aug-2016 15 400
034 26-Aug-2016 15 400
035 26-Aug-2016 15 400 All sample sent; it will be in 2mL screw-cap vial
036 26-Aug-2016 15 400
103 26-Aug-2016 15 2800
104 26-Aug-2016 15 2800
105 26-Aug-2016 15 2800
106 26-Aug-2016 15 2800 All sample sent; it will be in 2mL screw-cap vial
108 26-Aug-2016 15 2800

Katie sent this additional info in an email to Steven and me:

These C. virginica samples were exposed to control (400, 6 samples) and OA (2800, 5 samples) conditions for ~4 weeks at 15C. Gonad was carefully extracted by peeling back the outer membrane, flash frozen in liquid N, and placed in -80C (until today when we removed it). During sampling, it was difficult to get a lot of what we considered “pure” gonadal tissue. We sent you ~1/2 of the amount of tissue we have for all samples except for the two samples which were very low and we sent you all the tissue sample we have. Each should be about 10-20 mg of tissue, which I’m worried is not enough for MBD-BS seq. Fingers crossed.

Samples Received – Acropora cervicornis (Staghorn coral) DNA from Javier Casariego (FIU)

Received 62 coral (Acropora cervicornis) DNA samples from Javier Casariego at FIU.

Spreadsheet of samples and NanoDrop concentrations provided by Javier (converted to Google Sheet): A.cervicornis_DNA_Extractions(May_2017).xlsx

Samples were temporarily stored at 4c (in FTR 213) until I can perform global methylation assessment on them tomorrow.

Samples Received – Coral DNA from Jose M. Eirin-Lopez (Florida International University)

Steven received these coral DNA samples today. Here’s his post on Google Plus (stored @ 4C in FTR 213):

 

 

Here’s the email from Jose describing the samples:

“Dear Steven, the coral DNA samples were sent today by my student Javier (cc’ed here) to your lab. Here’s an excel attached with info for the samples including concentration and treatment of the coral from which they were extracted (N, nitrogen; NP, nitrogen+phosphorous; C, control).

Please let us know when you get these in the lab so we know all is fine!

thanks!

Chema”

Here’s the spreadsheet he sent (renamed for easier identification later on – original file sent was title DNA Qbit readings), uploaded to Google Drive:

20160615_coral_DNA_Qbit_readings.xls

Samples Received – C.gigas Tissue & DNA from Katie Lotterhos

Received 6 samples from Katie today. The box was labeled and stored @ -20C.

 

Here description of the samples, via email:

Lotterhos samples (gigas) arriving tomorrow

1) Mantle tissue samples of C. gigas were collected on 20140705 (source: Pipestem Inlet) by KEL
2) Extraction on 20141028 by VG using Qiagen DNAeasy Blood and Tissue Kit
3) Beadwash on 20150720 by VG using homemade sera-mag speed beads
4) Qubit 3.0 quantification on 20151206 by KEL and the following amounts were sent:

M1: 13 uL of 386 ug/mL
M2: 13.8 uL of 326 ug/mL
M3: 13.15 uL of 380 ug/mL (solution looked cloudy)