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  • Steven Roberts posted an update in the group Ecology of Infectious Marine Diseases [course] 3 years, 4 months ago

    A few have been asking about the Pycnopodia coelomocyte transcriptome we will be examining in week 3. @morgan-eisenlord can provide some more details (as she did the exposures) but this represents 6 individuals – three exposed to 0.2 filter homegenate from sick stars and three exposed to 0.2 filtered heat-killed homegenate from sick stars (control).
    The assembled transcriptome is @

    http://eagle.fish.washington.edu/whale/eimd_14/Phel_transcriptome_clc.fa

    You are welcome to start playing with it- let me know if you have any questions.

    • Thanks Steven! I will post a write up of the experiment when I get a chance. It will be done before we start on this data set.

      • The exposed stars were all in early disease stages. The control stars remained healthy. I will include photos for each star on the day samples were taken. We also ran qPCR on samples taken at the same time from these animals, as well as EM and histology.

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