Monthly Archives: October, 2015

  • DNA extraction 10/29/2015

    The six oyster DNA samples ( #27 – 31) incubated yesterday (10/28/2015) were taken out. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal Transplant Final Sampling’.  

  • DNA Extraction and Opening Oysters 10/28/2015

    The six oyster DNA samples ( #22-26) incubated yesterday (10/27/2015) were taken out. DNA was extracted from them using the mollusc DNA extraction kit. All the optional steps were excluded. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal Transplant Final Sampling’. Took out 6 oysters from Fidalgo Bay population for »more

  • Opening Oysters 10/27/2015

    Took out 5 oysters from Fidalgo Bay population for DNA extraction. I collected gill tissue from oysters #22 – 26 for DNA extraction. Gill tissues of all oyster were collected in MBL 1 buffer and proteinase K for DNA isolation by  mollusc kit. Placed all the tubes with gill tissues in 37 C for overnight. »more

  • DNA extraction from Oysters

    The six oyster DNA samples ( #15, 16, 18- 21) incubated yesterday (10/22/2015) were taken out. DNA was extracted from them using the mollusc DNA extraction kit. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal Transplant Final Sampling’.

  • Opening oysters – 10/22/2015

    Took out 7 oysters from Fidalgo Bay population for DNA extraction. I collected gill tissue from oysters #15 – 21 for DNA extraction (Oyster #17 was dead.). Gill tissues of all oyster were collected in MBL 1 buffer and proteinase K for DNA isolation by  mollusc kit. Placed all the tubes with gill tissues in »more

  • Opening Oysters

    Took out 12 oysters from Fidalgo Bay population for DNA extraction. I collected gill tissue from oysters #4 – 8 for DNA extraction. Gill tissues of all oyster were collected in MBL 1 buffer and proteinase K for DNA isolation by  mollusc kit. Viscera with digestive glands and gonads, etc. were dissected and discarded to »more

  • DNA extraction from oysters

    Took 6 oysters from 4SN population collected at Manchester for DNA extraction —> Found all dead. We have weighed and photo documented them for length/width measurements. Took two oysters randomly from 2SN population collected at Fidalgo bay (and they were both alive.). Weighed and photo documented them for length/width measurements. I learned how to open »more