Took out 12 oysters from Fidalgo Bay population for DNA extraction.
I collected gill tissue from oysters #4 – 8 for DNA extraction.
Gill tissues of all oyster were collected in MBL 1 buffer and proteinase K for DNA isolation by mollusc kit.
Viscera with digestive glands and gonads, etc. were dissected and discarded to prevent decomposition of rest of body by enzymes. Rest of the body of each oyster was stored in 10X RNAlater solution by weight.
Weighed and recorded following for each oyster:
1) Complete organism
2) Gill tissue collected
3) Body parts collected for storage (excluding viscera)
4) Empty shells