Tag Archives: 2SN

  • DNA Extraction 12/2/15

    The four oyster DNA samples ( # 100, 2HL 1 – 3) incubated at 60 degree for an hour. Samples were previously stored at -80 degrees. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA »more

  • DNA Extraction – 11/19/15

    The six oyster DNA samples ( # 94 – 99) incubated at 60 degree for an hour. Samples were previously stored at -80 degrees. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal »more

  • DNA Extraction – 11/18/15

    The six oyster DNA samples ( # 88 – 93) incubated at 60 degree for an hour. Samples were previously stored at -80 degrees. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal »more

  • DNA extraction- 11/17/15

    The six oyster DNA samples ( # 82 – 87) incubated at 60 degree for an hour. Samples were previously stored at -80 degrees. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal »more

  • DNA Extraction 11/13/15

    The six oyster DNA samples ( #76 – 81) incubated at 60 degree for an hour. Samples were previously stored at -80 degrees. We tried some steps to increase the yield: 1) Centrifuge the sample before adding isopropylalcohol to settle down the tissue. Take only the liquid for rest of the protocol – Didn’t work »more

  • DNA Extraction 11/10/15

    The eight oyster DNA samples ( #68 – 75) incubated at 60 degree. Samples were previously stored at -80 degrees. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal Transplant Final Sampling’.

  • DNA extraction 10/29/2015

    The six oyster DNA samples ( #27 – 31) incubated yesterday (10/28/2015) were taken out. DNA was extracted from them using the mollusc DNA extraction kit. None of the optional steps were performed. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal Transplant Final Sampling’.  

  • DNA Extraction and Opening Oysters 10/28/2015

    The six oyster DNA samples ( #22-26) incubated yesterday (10/27/2015) were taken out. DNA was extracted from them using the mollusc DNA extraction kit. All the optional steps were excluded. Later the samples were stored in refrigerator in box ‘Oly gDNA Oly Reciprocal Transplant Final Sampling’. Took out 6 oysters from Fidalgo Bay population for »more

  • Opening Oysters 10/27/2015

    Took out 5 oysters from Fidalgo Bay population for DNA extraction. I collected gill tissue from oysters #22 – 26 for DNA extraction. Gill tissues of all oyster were collected in MBL 1 buffer and proteinase K for DNA isolation by  mollusc kit. Placed all the tubes with gill tissues in 37 C for overnight. »more

  • DNA extraction from oysters

    Took 6 oysters from 4SN population collected at Manchester for DNA extraction —> Found all dead. We have weighed and photo documented them for length/width measurements. Took two oysters randomly from 2SN population collected at Fidalgo bay (and they were both alive.). Weighed and photo documented them for length/width measurements. I learned how to open »more