DNA Extraction – LCM Samples from 20100217

Used the Pico Pure DNA Extraction Kit to extract DNA from laser capture microdissection samples (provided by Lisa; stored in -20C in Rm. 236) from 20100217 (New 08-1-702.17.10; Class 08:13-5a 2.17.10) according to the manufacturer’s protocol. Briefly, a single aliquot of lyophilized Proteinase K was reconstituted in 155uL of the provided buffer. 50uL of this solution was added to two fresh 0.5mL snap cap tubes. The LCM caps containing were placed on these 0.5mL tubes, inverted and tapped to cover them in the Proteinase K solution. Tubes were incubated ~24hrs @ 65C.

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