DNA Amplification – LCM DNA from yesterday

Used the Genomiphi V2 Kit (Note: Expiration date on box listed as 05 Feb 2010!) according to the manufacturer’s protocol on both LCM DNAs extracted yesterday (New 08-1-702.17.10; Class 08:13-5a 2.17.10). Briefly, combined 1uL of LCM DNA with 9uL of Sample Buffer. Incubated 95C 3mins and then placed on ice. Added 9uL of Reaction Buffer and 1uL of Enzyme Mix to each sample. Incubated @ 30C for 1.5hrs, heated @ 65C for 10mins and then stored Genomiphi’d LCM DNA @ -20C.

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