Ran conventional PCR using RA 5-1 and RA 3-6 primers on the LCM and Genomiphi’d DNA isolated 20110413 and 20110414, respectively. Master mix calcs and cycling params are here.
Positive Control: “1:100 Plasmid RLP” from 7/24/09. This was supplied by Lisa to use as a positive control for these primers.
Well, the gel is totally and completely blank. Not even the positive control showed up. Will try to figure out what went wrong…
–UPDATE– Found original cycling parameters for this PCR in one of Lisa’s notebooks. Will try those when I repeat this.